Viral load estimation in asymptomatic honey bee colonies using the quantitative RT-PCR technique - Agropolis Accéder directement au contenu
Article Dans Une Revue Apidologie Année : 2007

Viral load estimation in asymptomatic honey bee colonies using the quantitative RT-PCR technique

Résumé

Honey bee (Apis mellifera L.) colonies are subjected to many persistent viral infections that do not exhibit clinical signs. The identification of criteria that could identify persistent or latent infections in bee colonies is a challenging task for field investigators and beekeepers. With this aim in view, we developed a molecular method to estimate the viral loads for six different RNA viruses in bee and mite individuals collected from seemingly healthy colonies (360 colonies). The data showed very large viral titres in some samples (> 10(9) copies per bee or mite). Discrepancies between adults and pupae viral RNA loads and, in several instances, significant seasonal variations among viruses were observed. The high titres of some RNA viruses recorded in mites confirm that Varroa destructor could promote viral infections in colonies
Fichier principal
Vignette du fichier
21101_20081223041440951_1.pdf (193.86 Ko) Télécharger le fichier
Origine : Fichiers éditeurs autorisés sur une archive ouverte
Loading...

Dates et versions

hal-02660237 , version 1 (30-05-2020)

Licence

Copyright (Tous droits réservés)

Identifiants

Citer

Laurent Gauthier, Diana Tentcheva, Magali Tournaire, Benjamin Dainat, Francois F. Cousserans, et al.. Viral load estimation in asymptomatic honey bee colonies using the quantitative RT-PCR technique. Apidologie, 2007, 38 (5), pp.426-435. ⟨10.1051/apido:2007026⟩. ⟨hal-02660237⟩
18 Consultations
36 Téléchargements

Altmetric

Partager

Gmail Facebook X LinkedIn More