Accéder directement au contenu Accéder directement à la navigation
Article dans une revue

Development of tools to rapidly identify cryptic species and characterize their genetic diversity in different European kelp species

Abstract : Marine ecosystems formed by kelp forests are severely threatened by global change and local coastline disturbances in many regions. In order to take appropriate conservation, mitigation, and restoration actions, it is crucial to identify the most diverse populations which could serve as a “reservoir” of genetic diversity. This requires the development of specific tools, such as microsatellite markers to investigate the level and spatial distribution of genetic diversity. Here, we tested new polymorphic microsatellite loci from the genome of the kelp, Laminaria digitata, and tested them for cross-amplification and polymorphism in four closely related congeneric species (Laminaria hyperborea, Laminaria ochroleuca, Laminaria rodriguezii, and Laminaria pallida). Adding these 20 new microsatellite loci to the ten L. digitata loci previously developed by Billot et al. (Mol Ecol 7:1778-1780, 1998) and Brenan et al. (J R Soc Interface 11:1-12, 2014) and to the ten L. ochroleuca loci previously developed by Coelho et al. (Conserv Genet Resource 6:949-950, 2014), we retained a total of 30 polymorphic loci for L. digitata, 21 for L. hyperborea, 16 for L ochroleuca, 18 for L. rodriguezii, and 12 for L. pallida. These markers have been tested for the first time in the last two species. As predicted, the proportion of markers that cross-amplified between species decreased with increasing genetic distance. In addition, as problems of species identification were reported in this family, mainly between L. digitata and Hedophyllum nigripes, but also between L. digitata, L. hyperborea, and L. ochroleuca in areas where their range distributions overlap, we report a rapid PCR identification method based on species-specific COI mitochondrial primers that allows these four species of kelp to be rapidly distinguished.
Liste complète des métadonnées
Contributeur : Hal Sorbonne Université Gestionnaire Connectez-vous pour contacter le contributeur
Soumis le : mardi 30 novembre 2021 - 09:59:53
Dernière modification le : mercredi 1 décembre 2021 - 03:08:04


Version_public (1).pdf
Fichiers produits par l'(les) auteur(s)



Stéphane Mauger, Louise Fouqueau, Komlan Avia, Lauric Reynes, Ester Serrao, et al.. Development of tools to rapidly identify cryptic species and characterize their genetic diversity in different European kelp species. Journal of Applied Phycology, Springer Verlag, 2021, 33 (6), pp.4169 - 4186. ⟨10.1007/s10811-021-02613-x⟩. ⟨hal-03375921⟩



Consultations de la notice